• br Methodology To simulate chip flow trajectory the force

  2020-06-15

  

  Methodology To simulate chip flow trajectory, the force and torque acting on the chips in high pressure coolant are computed based on the control volume method [6]. During the setup, a typical gun drill chip and gun drill bit are imported into ANSYS CFX 14.0 and prescribed as stationary solids. T

• Cultured cells or mouse lung tissues were harvested and

  2020-06-15

  

  Cultured cells or mouse lung tissues were harvested and lysed in Radio-Immunoprecipitation Assay (RIPA) buffer (0.05 mol/l Tris–HCl pH 7.4, 0.15 mol/l NaCl, 0.25% deoxycholic acid, 1% NP-40, 1 mmol/l ethylenediamine tetraacetic Chlorpropamide (EDTA), 1 mmol/l phenylmethylsulfonyl fuoride, 1 μg/ml

• It is well known that CysLTs

  2020-06-15

  

  It is well known that CysLTs exert potent contractile action on human bronchial smooth muscles through activation of CysLT1 receptors. Indeed, in vitro antigen-induced contraction of passively sensitized human bronchial tissue is markedly suppressed by a pre-treatment with CysLT1 receptor antagonist

• The data presented above clearly indicate an important role

  2020-06-15

  

  The data presented above clearly indicate an important role of eIF4E for the nucleocytoplasmic transport of the human iNOS mRNA. To analyze the sequence elements of the iNOS mRNA that are important for this eIF4E-mediated transport we performed transient transfection experiments using constructs con

• Immunofluorescence Cells were fixed with paraformaldehyde PF

  2020-06-15

  

  Immunofluorescence. Cells were fixed with 4% paraformaldehyde (PFA) in PBS and extracted by 0.5% Triton X-100-PBS, or fixed in cold methanol. The cells were immunostained with mouse monoclonal Cy5 RNA against α-tubulin (Sigma), γ-tubulin (Sigma), Ran (Upstate), rabbit polyclonal antibodies against

• Another question concerns the mechanism

  2020-06-15

  

  Another question concerns the mechanism of PC reduction during the phosphate starvation response. There are at least three main mechanisms of PC depletion: suppression of PC synthesis due to substrate exhaustion, downregulation of PC synthase expression by suppression of gene transcription or intens

• No effect on the A production by secretase was

  2020-06-15

  

  No effect on the Aβ production by γ-secretase was observed at 50μM, the highest effective concentration used in cultured C-type natriuretic peptide (1-22) (human, rat, swine) by the study of Flajolet et al. IC261 did also not exert a modulatory effect on the production of Aβ, Aβ and Aβ species in th

• As illustrated in Table monocyclic acid analogs were

  2020-06-15

  

  As illustrated in Table 3, monocyclic Okadaic acid analogs were synthesized and evaluated. 2-Oxido-3H-1,2,3,5-oxathiadiazol analog 8 showed 15-fold less potent EP1 receptor affinity relative to 2b, while it showed 2.2-fold more potent antagonist activity. Oxadiazole-5-one analog 9 exhibited nearly

• Enolase as a plasminogen receptor and activator localized

  2020-06-15

  

  Enolase, as a plasminogen receptor and activator localized on the surface of several helminths, can interact with the host fibrinolytic system (Yang et al., 2010, Du et al., 2009, de la Torre-Escudero et al., 2010). The enzyme is also one of excretory/secretory (ES) products in some helminths, such

• In the previous study glutamyl endopeptidase

  2020-06-15

  

  In the previous study glutamyl endopeptidase of B. intermedius has been reported to be distributed in the AT7519 receptor of B. intermedius as follows: 90% of synthesized enzyme has been detected in the culture liquid, 9.3% of glutamyl endopeptidase—in the fraction of membrane proteins [20]. The di

• The expression of cytokine inducible

  2020-06-15

  

  The expression of cytokine-inducible genes is mediated by a number of components within the cell. It has been shown that Jak2 and the Ras/Raf/MAP kinase signaling cascades are required for the expression of DUB-1[9], [29]. However, the enhancer domain of DUB-1 lacks a consensus sequence for Stat bin

• The fact that the mean tumor size of the E

  2020-06-15

  

  The fact that the mean tumor size of the E1202-654 + α-GalCer group was much smaller than that of the α-GalCer inoculated group in both protocols indicates that immunization with E1202-654 plus α-GalCer, which induces a strong CD8+ T cell E1 antigen-specific immune response, is involved in the elimi

• Primases are classified into two major groups first the

  2020-06-15

  

  Primases are classified into two major groups: first, the DnaG primases, found in bacteria and bacteriophages, and second, the archaeoeukaryotic primases. Remarkably, bacterial and archaeoeukaryotic primases have no structural similarity and, presumably, evolved independently (Leipe et al., 1999). T

• Structural homology modelling Intensive Phyre

  2020-06-15

  

  Structural homology modelling. Intensive Phyre2 modelling [44] was performed using the primary amino Cyclo sequence of A1S_0222 as input to generate an atomistic 3D-homology model of A1S_0222. The fit to the SAXS data of the homology model as well as the fit to the data of the E. coli adenine-N6-DN

• The outcome of DGK function will thus depend

  2020-06-15

  

  The outcome of DGK function will thus depend on the binding partners of each DGK isoform, and the effects that they exert can be quite different. A clear example of this concept has been demonstrated for the type IV DGKs, ζ and ι, which are structurally very similar but have opposing effects on Ras

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